Isolasi dan Karakterisasi Bakteri Tanah Sawah di Kecamatan Medan Satria dan Bekasi Utara, Kota Bekasi, Jawa Barat
DOI:
https://doi.org/10.36722/sst.v3i4.233Abstract
Abstrak - Produksi padi di Indonesia setiap tahun mengalami peningkatan, namun peningkatan ini belum mampu memenuhi kebutuhan nasional sehingga impor masih harus dilakukan. Salah satu masalah dalam produksi beras adalah penggunaan pupuk berlebih yang tidak hanya meningkatkan biaya produksi, namun juga merusak kondisi tanah. Aplikasi bakteri tanah sebagai Plant Growth Promoting Rhizobacteria (PGPR) dapat menjadi salah satu solusi terhadap masalah ini. Penelitian ini bertujuan untuk mengisolasi bakteri tanah dari 3 lokasi sawah daerah Bekasi, membandingkan keberadaan total bakteri pada ketiga lokasi tersebut, dan melakukan karakterisasi isolat berdasarkan karakter yang dapat memicu pertumbuhan tanaman. Dari ketiga lokasi, diperoleh total 59 isolat dan 5 diantaranya berpotensi sebagai PGPR karena kemampuan fiksasi Nitrogen, melarutkan Fosfat, katalase positif, dan motil. Dari ketiga lokasi pengambilan sampel, BK1 memiliki jumlah total bakteri terendah karena aplikasi pemupukan dan pestisida berlebih yang ditandai tingginya kadar P total, serta tingginya residu klorpirifos, karbofuran, dan paration. Kondisi fisik tanah BK1 juga didominasi partikel liat yang menyebabkan tanah menjadi lebih padat. Peningkatan jumlah penggunaan pupuk tidak selalu diikuti peningkatan produktivitas tanaman.
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Kata Kunci - Bakteri tanah, Rhizosfer sawah, PGPR, Pupuk Hayati
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Abstract - Rice production in Indonesia has increased annually, but this increase has not reached national demand,so imports still done. One of the problems in rice production is the use of excessive fertilizers that not only increase production costs, but also decreased the soil conditions. The application of soil bacteria as Plant Growth Promoting Rhizobacteria (PGPR) can be the one solution to face this problem. The objective of this study was isolate soil bacteria from 3 locations of rice field in Bekasi, compare the total bacteria in the three locations, and characterize isolates based on the character that can promote plant growth. From three locations, a total of 59 isolates were obtained and 5 of them were potential as a PGPRs due to its Nitrogen fixation activity, Phosphate solubilization, positive catalase, and motility. From three sampling sites, BK1 has the lowest TPC value because of excessive fertilizers and pesticides application which indicated by high total P levels, and also high chlorpyrifos, carbofuran and paration residues. The physical condition of BK1 soil is also dominated by clay particles which causes the soil more solid. Increasing of fertilizer application is not always followed by increased plant productivity.
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Keywords - Biofertilizer, PGPR, Rice field rhizosphere, Soil Bacteria
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