Cemaran Mikroba Pada Telur Penyu Sisik (Eretmochelys imbricata) di Pulau Kelapa Dua, Taman Nasional Laut Kepulauan Seribu, DKI Jakarta

Authors

  • Mukti Ageng Wicaksono Universitas Al Azhar Indonesia
  • Fitriani Nurhasanah UIN Syarif Hidayatullah
  • Dewi Elfidasari Universitas Al Azhar Indonesia
  • Irawan Sugoro Pusat Aplikasi Isotop dan Radiasi-BATAN

DOI:

https://doi.org/10.36722/sst.v4i2.266

Abstract

Abstrak – Penyu sisik (Eretmochelysimbricata) merupakan salah satu jenis penyu yang hidup di perairan Indonesia. Populasi penyu sisik saat ini terus mengalami penurunan. Salah satu faktor penyebabnya adalah keberadaan mikroba pencemar pada telur penyu. Tujuan dari penelitian ini adalah mengetahui cemaran mikroba pada telur penyu sisik di Pulau Kelapa Dua. Analisis secara mikrobiologi meliputi total bakteri, jamur, coliform, Enterobacter, dan Salmonella-Shigella dilakukan terhadap sampel telur segar dan telur gagal menetas. Hasil analisis menunjukkan bahwa semua sampel tercemar mikroba yang mempengaruhi daya tetas telur. Sampel cangkang telur segar memiliki total bakteri 9,60x105 CFU/butir,  jamur 2,35x105 propagul/butir, coliform 3,96x105 CFU/butir, Enterobacter 3,02x105 CFU/butir dan Salmonella-Shigella 1,68x105 CFU/butir, sedangkan hasil analisis dari sampel isi telur segar diperoleh total bakteri 1,60x104CFU/ml, jamur 3,00x102propagul/ml, coliform 2,00x105CFU/ml, Enterobacter 1,20x105CFU/ml dan Salmonella-Shigella 1,46x105CFU/ml. Hasil analisis dari sampel cangkang telur gagal menetas diperoleh total bakteri 7,20x106CFU/butir, jamur 1,92x105 propagul/butir, coliform 4,08x105 CFU/butir, Enterobacter 2,59x105CFU/butir dan Salmonella-Shigella 3,31x105CFU/butir, sedangkan hasil analisis dari sampel isi telur gagal menetas diperoleh total bakteri 1,00x108CFU/ml, jamur 3,50x105propagul/ml, coliform 5,00x103CFU/ml, Enterobacter 4,00x103CFU/ml dan Salmonella-Shigella 3,00x103CFU/ml.

Kata Kunci - Penyu sisik, telur, Pulau Kelapa Dua, cemaran mikroba.

 

Abstract - Hawksbill (Eretmochelysimbricata) is one of the turtle species that live in Indonesian waters. The current population of hawksbill continues to decline. One of the contributing factors is the presence of contaminant microbes in turtle eggs. The purpose of this research is to know microbial contamination on hawksbill eggs in Kelapa Dua Island. Microbiological analyzes included total bacteria, fungi, coliform, Enterobacter, and Salmonella-Shigella were performed on fresh egg samples and eggs failed to hatch. The results showed that all samples were contaminated with microbes affecting the hatchability of the eggs. Fresh eggshell samples had total bacteria of 9.60x105 CFU/grains, 2.35x105 fungus propagules/grains, coliform 3.96x105 CFU/grains, Enterobacter 3.02x105 CFU/grains and Salmonella-Shigella 1.68x105 CFU/grains, while yield analysis of fresh egg contents samples obtained total bacteria 1.60x104 CFU/ml, mushrooms 3,00x102 propagules/ml, coliform 2.00x105 CFU/ml, Enterobacter 1,20x105 CFU/ml and Salmonella-Shigella 1,46x105 CFU/ml. Results of analysis of eggshell samples failed to hatch obtained total bacteria 7.20x106 CFU/grains, 1.92x105 fungi propagules/grains, coliform 4.08x105 CFU/grains, Enterobacter 2.59x105 CFU/grains and Salmonella-Shigella 3.31x105 CFU/while the results of the analysis of the sample of egg contents failed to hatch obtained total bacteria 1.00x108 CFU/ml, mushrooms 3,50x105 propagules/ml, coliform 5.00x103 CFU/ml, Enterobacter 4,00x103 CFU/ml and Salmonella-Shigella 3, 00x103 CFU/ml.

 

Keywords – Hawksbill, Egg, Kelapa Dua Island, microbial contamination

Author Biographies

Mukti Ageng Wicaksono, Universitas Al Azhar Indonesia

Program Studi Biologi, Fakultas Sains dan Teknologi

Fitriani Nurhasanah, UIN Syarif Hidayatullah

Program Studi Biologi, Fakultas Sains dan Teknologi

Dewi Elfidasari, Universitas Al Azhar Indonesia

Program Studi Biologi, Fakultas Sains dan Teknologi

References

Al-Bahry SN, Mahmoud IY, Al-Harthy A, Elshafie AE, Al-Ghafri S, Alkindi AYA, Al-Amri I. 2004.Bacterial Contamination in freshly laid egg green turtles Chelonia mydas at Ras Al Hadd reserve, Oman. In: 24th International Symposium for Sea Turtles. San Jose, Costa Rica.

Al-Bahry S, Mahmoud I, Elshafie A, Al-Harthy A, Al-Ghafri S, Al-Amri I, Alkindi A. 2009. Ultrastruktural features and elemental distribution in eggshell during pre and post hatching periods in the green turltle, Chelonia mydas at Ras Al-Hadd, Oman. Tiss Cell. 41:214-221.

Al-Bahry S, Mahmoud I, Elshafie A, Al-Harthy A, Al-Zadjali M, Al-Alawi W. 2011. Antibiotic resistant bacteria as bioindicator of polluted effluent in the green turtle, Chelonia mydas in Oman. Mar Envir Res. 71:139-144.

Estika A. 2013. Analisis mikroorganisme pada telur penyu hijau (Chelonia mydas) dari Pulau Bilang-bilangan, Kalimantan Timur. Prosiding Seminar Nasional Biologi. UPI. Bandung.

Foti M, Giacopello C, Bottari T, Fisichella V. Rinaldo D, Mammina C. 2009. Antibiotic resistance of gram negatives isolates from loggerhead sea turtle (Caretta caretta) in the central Mediteranian Sea. Mar Poll Bull. 58:1363-1366.

Gans C & Gaunt AS. 1969. Shell and physiology of turtles. Afr. Wildlife. 23:197-206.

Janawi. 2009. Perkembangan Suhu Sarang Penetasan Buatan pada Penetasan Telur Penyu hijau (Chelonia mydas L.) di Pantai Pangumbahan Kabupatan Sukabumi. [skripsi]. Fakultas Pertanian Universitas Suryakencana. Cianjur.

Keene . 2012. Microorganisms from Sand, Cloacal Fluid, and Eggs of Lepidochelys olivacea and Standard Testing of Cloacal Fluid Antimicrobial Propertie. Master Thesis. University of Indiana.

Lam J. 2006. Levels of trace elements in green turtle eggs collected from Hong Kong. Environ Poll. 144:790-801.

Nur N. 2004. Sea Turtle Conseation in Malaysia. www.wildasia.net (Diakses pada 30 Januari 2014].

Rudiana E, Ismunarti D.H, Nirwani S. 2004. Tingkat Keberhasilan Penetasan dan Masa Inkubasi Telur Penyu Hijau, Chelonia mydas L pada Perbedaan Waktu Pemindahan. Ilmu Kelautan.UNDIP. Semarang.

Spotilla JR. 2004. Sea Turtles: A Complete Guide to Their Biology, Behavior, and Conservation. John Hopkins University. Baltimore.

Suwelo IS, Widodo SR, Ating S. 1992. Penyu Sisik di Indonesia. LIPI Oseanografi. Jakarta. Jurnal Oseana. 17: 97-109.

Syamsuni YF. 2006. Karakteristik Habitat dan Penyebaran Sarang Penyu sisik (Eretmochelys imbricata). [Skripsi]. Jurusan Ilmu Kelautan. Fakultas Perikanan dan Ilmu Kelautan. Institut Pertanian Bogor. Bogor.

Phillott AD. 2002. Fungal colonization of sea turtle nests in estern australia. Ph.D Dissertation. Central Queensland University.

Phillot AD, Parmenter CJ, McKillup SC. 2006. Calcium depletion of eggshell after fungal invasion of sea turtle eggs. Chelon Conserv Biol. 5:146-149.

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Published

2018-01-31

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Section

JURNAL AL-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI