Patogenitas Nematoda Entomopatogen Heterorhabditis spp. Terhadap Larva Spodoptera litura

Authors

  • Hawwa' Cahya Maulida Universitas Islam Negeri Sunan Ampel Surabaya
  • Saiku Rokhim Universitas Islam Negeri Sunan Ampel Surabaya
  • Erna Zahro’in Balai Besar Perbenihan dan Proteksi Tanaman Perkebunan Surabaya

DOI:

https://doi.org/10.36722/sst.v6i2.809

Abstract

Spodoptera litura is a pest in vegetable plants that attacks the leaves and stems. Damage arising from the attack of S. litura can decrease the productivity of plants. Synthetic pesticides are often used in controlling the population of S. litura, but synthetic pesticides have a high negative impact. Potential entomopathogenic nematode Heterorhabditis spp. believed to be effective for controlling pest populations. This study aims to find out the pathogenic value of entomopathogenic nematode Heterorhabditis spp. to the larvae of S. litura. Based on the results obtained Heterorhabditis spp. has a positive influence in causing mortality in the larvae of S. litura. Heterorhabditis spp. mortality of up to 42%. Symptoms indicated by larvae of S. litura affected by Heterorhabditis spp. among them the behavior becomes passive, the body becomes flaccid, the cuticle turns red and the tissues inside the body are destroyed. Obtained pathogenicity value Lc 50 Heterorhabditis spp. 7,690 IJ/ml, as for the factors affecting Heterorhabditis spp. such as humidity, temperature, pH, and light intensity.

Keywords – Entomopathogenic Nematodes, Pathogenicity, Spodoptera litura

Author Biographies

Hawwa' Cahya Maulida, Universitas Islam Negeri Sunan Ampel Surabaya

Program studi Biologi, Fakultas Sains dan Teknologi

Saiku Rokhim, Universitas Islam Negeri Sunan Ampel Surabaya

Program studi Biologi, Fakultas Sains dan Teknologi

References

F. Dwimartina, R. Rostaman, dan L. Soesanto, “Keefektifan Bakteri Serratia Endosimbion WBC Terhadap Ulat Grayak (Spodoptera litura F.) Di Laboratorium Entomologi BBPOPT Jatisari Karawang,†Agro Wiralodra, vol. 3, no. 1, hal. 29–35, 2020, doi: 10.31943/agrowiralodra.v3i1.39.

Z. A. Amin, T. Wardhani, dan S. Pratamaningtyas, “Pengaruh Metode Maserasi Jazzar Dan Balafif Dalam Memperoleh Ekstrak Air Daun Mindi (Melia azedarach L.) Sebagai Insektisida Botani Pada Ulat Grayak (Spodoptera litura F.),†J. Pertan. 2016, vol. 10, no. 2, hal. 110–121, 2016.

Badaruddin, D. Fitriyanti, dan Susilawati, “Pelatihan Pembuatan Pestisida Hayati Ramah Lingkungan Di Kampung Sayur Kelurahan Landasan Ulin Utara Banjarbaru,†in Prosiding Seminar Nasional Pengabdian Kepada Masyarakat, 2020, vol. 2, hal. 15–20.

A. Ibrahim, Yulistiawati A.S. Jasil, A. Rosyad “Agen Hayati Pemacu Pertumbuhan Dan Pengendali Penyakit Tertular Benih Beberapa Tanaman Pangan Dan Hortikulurai,†Bul. Inov. Pertan. Spesifik Lokasi, vol. 6 no. 2, hal. 169–76, 2019.

H. Afifah, L., Rahardjo B.T. dan Tarno, “Eksplorasi Nematoda Entomopatogen pada Lahan Tanaman Jagung, Kedelai, dan Kubis di Malang serta Virulensinya terhadap Spodoptera litura Fabricius,†J. HPT, vol. 1, no. 2, hal. 1–9, 2013.

Suyadi, Rosfiansyah, J. Nurdiana, A. Suryadi, Sopialema dan S.Waluyo “Studi Genera Nematoda Entomopatogen Pada Lahan Lebak Padi Sawah (O r y z a s a t i v a L.) Di Kecamatan Muara Wis Kabupaten Kutai Kartanegara,†in Konferensi Antarabangsa Islam Borneo Ke-10 2017, 2017, September, hal. 500–507.

G. Indriati and I. Trisawa, “Nematoda Patogen Serangga Heterorhabditis spp. Untuk Pengendalian Hama Penggerek Batang Lada,†J. Ind. Beverage Crop., vol. 2, no. 3, hal. 291–296, 2011.

W. Widayati, “Uji Efikasi Nematoda Entomopatogen Pada Hama Tanaman Cabai,†Agritrop J. Ilmu-Ilmu Pertan., hal. 63–66, 2012.

S. Khairunnisa, M. I. Pinem. dan F. Zahra “Uji Efektifitas Nematoda Entomopatogen Sebagai Pengendali Penggerek Pucuk Kelapa Sawit (Oryctes rhinoceros L.) (Coleoptera: Scarabaidae) Di Laboratorium,†J. Online Agroekoteknologi, vol. 2, no. 2, hal. 1–2, 2014.

B. T. Rahardjo, H. Tarno, and L. Afifah, “Efikasi Nematoda Entomopatogen Heterorhabditis sp. isolat Lokal Terhadap Diamond Back Moth Plutella xylostella,†J. HPT, vol. 2, no. 2, hal. 1–8, 2014.

S. Shan. H. Ma, Y. Li, C. Huang, X. Gu, Z. Jiang, B. Sun, C. Chen, X. Wei, G. Shen. D. Shapiro-Ilan dan W. Ruan., “Metabolites From Symbiotic Bacteria Of Entomopathogenic Nematodes Have Antimicrobial Effects Against Pythium myriotylum,†Eur. J. Plant Pathol., vol. 158, no. 1, pp. 35–44, 2020, doi: 10.1007/s10658-020-02053-2.

F. L. Inman, S. Singh, dan L. D. Holmes, “Mass Production of the Beneficial Nematode Heterorhabditis bacteriophora and Its Bacterial Symbiont Photorhabdus luminescens,†Indian J. Microbiol., vol. 52, no. 3, pp. 316–324, 2012, doi: 10.1007/s12088-012-0270-2.

P. Widiyaningrum, N. dan Subekti, dan B. Priyono, “Uji Patogenitas Nematoda Entomopatogen Isolat Semarang Steinernema sp Pada Rayap Tanah Macrotermes sp,†in Prosiding Semnas Hasil Penelitian., 2016, hal. 178–182.

A. B. Satria, P. Widiyaningrum, and S. Ngabekti, “Viabilitas Dua Isolat Lokal Nematoda Entomopatogen pada Berbagai Variasi pH,†Life Sci., vol. 7, no. 1, hal. 9–15, 2018.

M. Zart et al., “Performance of Entomopathogenic Nematodes On The Mealybug, Dysmicoccus brevipes (Hemiptera: Pseudococcidae) And The Compatibility Of Control Agents With Nematodes,†J. Nematol., vol. 53, pp. 1–10, 2021, doi: 10.21307/JOFNEM-2021-020.

M. Lortkipanidze, K. Hwseynov, M. Kokhia, O. Gorgadze, and M. Kuchava, “Effect of Temperature on the Virulence of Entomopathogenic Nematodes,†Adv. Ecol. Environ. Res., pp. 32–38, 2019.

I. G. A. A. Indrayani and S. dan Chaerani, “Patogenisitas Nematoda Entomopatogen Terhadap Hama Uret Tebu Lepidiota stigma (Coleoptera : Scarabaeidae),†Bul. Plasma Nutfah, vol. 24, no. 2, hal. 83–88, 2018.

D. R. Indriyanti, N. F. Awalliyah, and P. Widiyaningrum, “Perbanyakan Nematoda Entomopatogen ( Nep ) Pada Berbagai Media Buatan Entomopathogenic Nematodes (Enps) Rearing,†Sainteknol J. Sains Dan Teknol., vol. 13, hal. 9–16, 2015.

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Published

2021-09-27

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JURNAL AL-AZHAR INDONESIA SERI SAINS DAN TEKNOLOGI