Optimization of Benzene and Toluene Biodegradation by Aspergillus niger and Phanerochaete chrysosporium
DOI:
https://doi.org/10.36722/sst.v5i2.355Abstract
Abstract - Benzene and toluene can cause contamination in environments. Utilization of microorganism to degrade these compounds could be favorable approach to discover a convenient biodegradation agent. Fungi have an important role in the degradation of petroleum hydrocarbon (PHC). The aim of this study was to evaluate the potential of benzene and toluene degrading molds Aspergillus niger and Phanerochaete chrysosporium. These molds were selected to check the Lignin Peroxidase (LiPs) activity and formulate the medium conditions for improving degradation process. Optimization of medium conditions (pH and nutrient concentrations) for benzene and toluene degradation was applied under in vitro conditions. Molds were grown in sawdust media with pH settings (4, 5, 6 and 7) and nutrients (25%, 50%, 75% and 100%) followed by the addition of benzene and toluene. The percentage of degradation was analyzed using Gas Chromatography (GC). The optimal degradation conditions of benzene and toluene by A. niger were at pH 6 with nutrient concentration of 75%, while P. chrysosporium at pH 7 with nutrient concentration of 25%. All samples that had been polluted by benzene and toluene did not show LiPs activity, however non polluted samples showed LiPs activity 0,528 U/ml in A. niger and 0,275 U/mL in P. chrysosporium.
Keywords- Aspergillus niger, Benzene, Biodegradation, Phanerochaete chrysosporium, Toluene.
References
M. Gholami; H. R. Nassehinia; A.J. Jafari; S. Nasseri; A. Esrafili, "Comparison of benzene & toluene removal from synthetic polluted air with use of nano photocatalytic TiO2/ZNO process," J. Environ Health Sci Eng , vol. 12, no. 1, pp. 1-8, 2014.
The National Toxicology Program, "Report on Carcinogens (RoC), Research Triangle Park, pp 1-5," The National Toxicology Program, United State, 2005.
C. B. Chikere; C. C. Azubuike, "Characterization of hydrocarbon utilizing fungi from," J. Biotech, vol. 27 , p. 49 – 54 , 2014.
J S Yadav, C A Reddy, "Degradation of benzene, toluene, ethylbenzene, and xylenes (BTEX) by the lignin-degrading basidiomycete Phanerochaete chrysosporium.," American Society for Microbiology, vol. 59, no. 3, pp. 756 - 762, 1993.
S. Bhuvaneswari; A.G. Deepa Priya; A.R. Dhayananda Shakar; C.S. Sharath Kumar; M. Gopinath, "A Comparative Study on Removal of Toluene by," International Journal of Industrial Engineering and Management Science, vol. 2, no. 1, 2012.
M. T. Krik and T. Kent, "Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H2O2-requiring oxygenase," Proc. Natl. Acad. Sci, vol. 81, pp. 2280-2284, 1984.
B. Rani,V. Kumar, J. Singh, S. Bisht, P. Teotia, S. Sharma, R. Kela, "Bioremediation of dyes by fungi isolated from contaminated dye effluent sites for bio-usability.," Braz J Microbiol., vol. 45, no. 3, 2014..
I. M. Ilmi and N. D. Kuswytasari, "Aktifitas Enzim Lignin Peroksidase oleh Gliomastix sp. T3.7 pada Limbah Bonggol Jagung dengan Berbagai pH dan Suhu," Sains dan Seni Pomits, vol. 2, no. 1, 2013.
A. Kanti, "POTENSI KAPANG Aspergillus niger, Rhizopus oryzae DAN Neurospora sitophila," Buletin Peternakan, vol. 41, no. 1, 2017.
I.-G. Jung and C.-H. Park, "Characteristics of Rhodococcus pyridinovorans PYJ-1 for the Biodegradation of Benzene, Toluene, m-Xylene (BTX), and Their Mixtures," Journal of Bioscience and Bioengineering, vol. 97, no. 6, 2004.
D. L. T. Retno, N. Mulyana, N. Nurhasni and U. Hasanah, "Pengaruh Radiasi Sinar Gamma Terhadap Kemampuan Degradasi Lignin Phanerochaete Chrysosporium dan Ganoderma Lucidum," Jurnal Sains dan Teknologi Nuklir Indonesia, vol. 17, pp. 21 - 36, 2016.
K. Bishnoi, R. Kumar and R. Narsi, "Biodegradation of polycyclic aromatic hydrocarbons by white rot fungi Phanerochaete chrysosporium in sterile and unsterile soil," Journal of Scientific & Industrial Research, vol. 67, pp. 538 - 542, 2008.
I. GarcÃa-Peña, I. Ortizc, S. Hernández and S. Revah, "Biofiltration of BTEX by the fungus Paecilomyces variotii," International Biodeterioration & Biodegradation, vol. 62, no. 4, pp. 442 - 447, 2008.
K. Rajendran, M. A. A. Karim and M. S. M. Annuar, "Optimization of nutrient levels for laccase fermentation using statistical techniques," Asia-Pacific Journal of Molecular Biology and Biotechnology, vol. 19, no. 2, pp. 73-81, 2011.
I. Pramita, P. Periadnadi and N. Nurmiati, "Pengaruh Kapur dan Dolomit Terhadap Pertumbuhan Miselium dan Produksi Jamur Kuping Hitam (Auricularia polythrica (Mont.) Sacc.)," Natural Science, vol. 4, no. 3, 2015.
Sutarma, Kultur Media Bakteri, Bogor: Balai Penelitian Veteriner, 2000.
L. Zacchi, G. Burla, D. Zuolong and P. J. Harvey., "Metabolism of cellulose by Phanerochaete chrysosporium in continuously agitated culture is associated with enhanced production of lignin peroxidase.," Biotechnol, vol. 8, no. 2, 2000.
M. Ali, M. N. M. Abdelghany and N. Khalil, "Biodegradation of some polycyclic aromatic hydrocarbons by Aspergillus terreus," African journal of microbiology research , vol. 6, no. 16, 2012.
